Figure 4

Immunoprofiling of cortical periventricular layers after in utero electroporation of plasmids pPri-miR-124(2) and pPri-miR-155/neg_control; part I. (A, B) Distribution of terminally administered BrdU, Pax6, Tbr2, phospho-histone3 (PHH3) and pCMV-driven EmGFP on E14.5 mid-frontal sections from brains electroporated in utero at E12.5 with plasmids pPri-miR-124(2) and pPri-miR-155/neg_control. Arrowheads point to abventricular displaced cells expressing Pax6 and Tbr2. Insets illustrate examples of EGFP/marker co-localizations, which were the subject of counting and statistical analyses. (C) Fractions of EmGFP⁺ cells immunoreactive for BrdU, Pax6, Tbr2 and PHH3. *P < 0.05, as calculated by ANOVA test; N = 5+5 for BdU and Tbr2; N = 4+4 for Pax6; N = 3+3 for PHH3. Scale bars = 100 μm.