Figure 2

Comparative profiling of cortical periventricular layers for miR-124 and markers of apical progenitors (Pax6 and pTα1-driven EGFP), basal progenitors (Tbr2) and post-mitotic neurons (β-tubulin). (A) Schematic of an E14 midfrontal telencephalic section showing the areas analyzed. (B, E, H, K) Immunofluorescence of Pax6, pTα1-driven EGFP, Tbr2 and neuron-specific β-tubulin, respectively. (C, F, I, L) In situ hybridization of miR-124. (D, G, J, M) Electronic merging of (B, C), (E, F) (H, I) and (K, K), respectively. Numbered magnifications of boxed areas in (D, G, J, M) show Pax6, pTα1-EGFP, Tbr2 and β-tubulin in cells expressing different levels of miR-124 Arrowheads in (D1), (J1) and (M) point to Pax6^-/miR-124^high, Tbr2^-/miR-124^high and β-tubulin^-/miR-124^high elements, respectively. Double arrowheads in (G3) denote pTα1-EGFP⁺/miR-124^high cells that no longer contact the ventricular cavity. Arrows in (M1) indicate SVZ β-tubulin⁺ cells expressing intermediate levels of miR-124. Asterisks in (M2, M3) demarcate SVZ β-tubulin^- cells expressing high levels of miR-124. Scale bars = 100 μm.