Figure 6
From: Fgf receptor 3 activation promotes selective growth and expansion of occipitotemporal cortex
Regulation of proliferating progenitor cells in the EIIa-Cre;Fgfr3+/K644Ecortex at E18.5. (a-c) Time-mated females were injected with bromodeoxyuridine (BrdU) for 1 hour prior to termination at E18.5. Immunohistochemistry for Pax6/BrdU (b) and Tbr2/BrdU (c) were used to identify proliferating radial glia (arrows in (b)) and intermediate progenitor cell (IPCs; arrows in (c)) on consecutive sections. Panels are examples of the staining in wild type at the rostral region. The white dashed line in (b, c) indicates the apical surface of the VZ. Cells were counted within a 100 μm channel that spans the ventricular zone (VZ)/subventricular zone (SVZ), identified by Nissl (a) and by a region containing the majority of the Tbr2 immunoreactivity (c) (marked with the yellow dashed line). Three embryos per genotype and two counting boxes per samples were used (n = 6). Scale bar: 20 μm. (d) Total numbers of proliferating (BrdU+) cells in the VZ/SVZ in the dorsal cortical regions. (e) Total numbers of proliferating radial glia (BrdU+Pax6+) in the VZ. (f) Total numbers of IPCs (Tbr2+) in the VZ/SVZ. (g) Proportion of proliferating IPCs (BrdU+Tbr2+) within the proliferating population (BrdU+). (h) Proportions of proliferating radial glia (BrdU+Pax6+) within the Pax6+ population. (i) Proportions of proliferating IPCs (BrdU+ Tbr2+) within the IPC (Tbr2+) population. Asterisks *, **, and *** indicate p values < 0.05, < 0.005, and < 0.005 by Student's t- test, respectively; See Results for details.