Figure 1
From: Fgf receptor 3 activation promotes selective growth and expansion of occipitotemporal cortex
Rostral shift of cortical areas in Nestin-Cre;Fgfr3+/K644E. (a, b) Cortical areas were visualized in tangential sections of the flattened cortex of Nestin-Cre;Fgfr3+/K644E(b) and wild-type (Wt) littermates (a) by immunohistochemistry for the serotonin transport protein 5-hydroxy-tryptamine (5-HTT). In (a, b), cortical hemispheres at postnatal day (P)8.5 were dissected so that the primary somatosensory area (S1), including the posteromedial barrel subfield (PMBSF), was clearly displayed. Irregularity in the barrel pattern was observed in Nestin-Cre;Fgfr3+/K644E(b, arrow). (c) Position of the PMBSF was quantified by cytochrome C oxidase histochemistry performed at P6.5, using C4 subfield as a reference point, and presented as a frontal (F) and occipital (O) ratio to the total cortical length (T). (d-f) Measurements showed an increase in total and occipital length, while the frontal length remained similar. (g, h) The F/T and O/T length ratios clearly show that the PMBSF was rostrally shifted in Nestin-Cre;Fgfr3+/K644E. (i) Position of the PMBSF was quantified for the total (W), medial (M) and lateral (L) lengths. (j-l) The total and lateral lengths were increased in Nestin-Cre;Fgfr3+/K644Ecompared to wild type, while the medial length was similar. (m, n) The M/W and L/W ratios show that the PMBSF was medially shifted in Nestin-Cre;Fgfr3+/K644E. Scale bar: 1 mm in (a, b).