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Figure 3 | Neural Development

Figure 3

From: Temporal patterns of broad isoform expression during the development of neuronal lineages in Drosophila

Figure 3

The time-course of Br-Z3 expression in neuroblast MARCM clones induced after hatching. (A) Three examples of thoracic neuroblast clones for lineage 3 showing the change in Br-Z3 expression in neuronal populations through larval growth. Left: Z-projection of the clones showing the increase in overall size and morphology at 72 and 96 h AEL, and at puparium formation (P0). Right: sequential sections through each clone showing the distribution of Br-Z3+ neurons in the cluster. At 72 h AEL, Br-Z3 is barely detectable in larval born neurons; by 96 h AEL the basal cells (left) are Br-Z3+ but the more apical cells are negative. At P0 the basal cells (left) are now negative while more apical cells are Br-Z3+ except for those nearest the neuroblast (NB). (B, C) Use of BrdU pulses at 96 h AEL to establish the time of Br-Z3 expression relative to the end of DNA synthesis in the parent GMC. (B) Confocal sections through neuroblast clones for lineage 3 from larvae fed on a BrdU diet for 8 (top) or 16 h (bottom) prior to sacrifice. Br-Z3- and BrdU-labeled cells did not overlap after the 8 h pulse but did so after the 16 h pulse. Arrow points to the same nucleus in each panel. (C) The frequency of clones that had co-labeled cells as a function of the duration of the BrdU pulse. Numbers above each bar are the numbers of clones examined for each treatment.

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