Figure 2

Examples of early embryonic induced MARCM clones and showing embryonic-born secondary neurons and their state of Broad expression. Left: Z-projections of green fluorescent protein (GFP)-labeled clones. Right: individual confocal sections at various levels through the clone (section number) showing GFP-labeled neurons (green) and either BrdU or Br-Z3 (magenta). (A) Early embryonic induced neuroblast clone of thoracic lineage 9 from a third instar larva that had fed on a BrdU diet from the time of hatching. The large basal neurons in the clone lack BrdU incorporation, showing that they were born before hatching. The red-framed area is represented in the individual sections. (B) Three neuroblast clones in the lateral thorax at the start of the third larval instar (approximately 72 h after egg laying), showing that the basal neurons express Br-Z3 at this time. NB, neuroblast.