Figure 1
Immunocytochemistry showing staining with a MAb directed against the core region of the Broad transcription factor. (A, B) Whole mounts of Drosophila (A) and Manduca (B) embryos just prior to hatching. Broad expression is confined to the brain (b) and ventral CNS in both; mh, mouth hooks. (C) Confocal optical section through the thoracic and abdominal neuromeres of the CNS of a newly hatched larva of Drosophila that is double stained for the neuron-specific marker, Elav (magenta) and Broad (BR, green). All of the Broad-positive cells coincide with cells that also express Elav. Anterior is up; the CNS is slightly twisted so that only the right halves of the abdominal neuromeres are in the section. (D, E) Lateral views of Drosophila embryos at late stage 12 and stage 15, respectively, showing the expression of Broad confined to the developing CNS. (F) The CNS of a newly hatched larva lacking broad function (brnpr 3homozygote) showing no Broad immunostaining.