Multidendritic sensory neurons in the adult Drosophila abdomen: origins, dendritic morphology, and segment- and age-dependent programmed cell death

Table 1 MARCM analysis to trace the origins of adult dendritic arborization neurons back to the larval stage.

		Number of clones in third instar larvae	Number of clones in the adults
d	ddaA (class III)	9	0
	ddaB (class II)	8	0
	ddaC (class IV)	19	19
	ddaD (class I)	11	11^a
	ddaE (class I)	3	3^a
	ddaF (class III)	21	0
l	ldaA (class II)	6	6
	ldaB (class III)	9	1^b
v'	v'ada (class IV)	10	10
	v'pda (class III)	10	0
v	vpda (class I)	6	0
	vdaA (class II)	3	0
	vdaB (class IV)	4	0
	vdaC (class II)	4	1^b
	vdaD (class III)	2	0

We first generated MARCM clones, and recorded their cell identities and hemisegments where the clones were located in mature larvae. When the animals became adults, we mounted their abdomens and scored all of the marked cells in the recorded hemisegments. We excluded from counting all larvae in which multiple clones were marked in single hemisegments. d, l, v', and v represent dorsal, lateral, ventral prime, and ventral clusters, respectively. ^aClones were identified within 12 hours after eclosion. This was partly because Gal4^109(2)80-dependent GFP signals were difficult to find in ddaD at later stages, and partly because ddaE executed programmed cell death within 1 week after eclosion. ^bWe interpreted this clone to be ldaA-like that most likely had been derived from a cell other than larval dendritic arborization neurons (see details in the text).

ISSN: 1749-8104