Almost all ddaE neurons underwent apoptosis within 1 week after eclosion. (A-C) Whole-mounts of a 0- to 1-hour-old female of genotype NP2225 UAS-mCD8::GFP/CyO (A), a 7- to 8-day-old female of the same genotype (B), and a 7- to 8-day-old female of genotype NP2225 UAS-mCD8::GFP/CyO; UAS-p35/+ (C). Yellow arrows in (A) point to cell bodies of ddaE in A2 to A4, and blue arrows in (A-C) to those of dorsal bipolar dendrite (dbd) neurons. Yellow brackets in (C) indicate dendritic arbors of surviving ddaE. Scale bars: 50 μm. (D) The percentage of each hemisegment that had ddaE. Genotypes and ages of male adults examined are indicated, and the number of each hemisegment observed is given above each bar. Colored short lines just on the x-axis mean that no ddaE signal was found in the hemisegment examined. (E) Quantification of the total number of multidendritic (md) neurons (da plus dbd) in each tergite of Canton S (CS) and Gal4109(2)80UAS-mCD8::GFP/Gal4109(2)80UAS-mCD8::GFP (Gal109) in 0- or 7-day-old adults. Adults were dissected and stained for HRP and Elav as in Additional file 4B-FB-F; and the number of md neurons in each tergite was counted. Each value is expressed as mean ± standard deviation. The number of animals examined is indicated above each bar.