Figure 3

Overall dendritic arbors of dendritic arborization (da) neurons in hemisegments A3 and A4 of the pharate adult. A fillet preparation of a pharate adult male of genotype Gal4^109(2)80UAS-mCD8::GFP/Gal4^109(2)80UAS-mCD8::GFP was doubly stained with anti-GFP antibody (A) (green in (C)) and anti-HRP antibody (B) (magenta or white in (C)). (A-C) High-power immunohistochemical images of the boxed region in Figure 2B. (D) Dendrites, cell bodies, and axons of the da and dorsal bipolar dendrite (dbd) neurons were traced. We traced the origins of individual da neurons back to the larval stage (described later in Figure 4). ldaA and ldaA-like are a pair of closely associated cells (Figure 5G-M). The position of ddaC relative to dbd along the dorsal-ventral axis is not fixed (see text). Cell bodies of the da neurons were located more deeply than their dendrites. The pleura included GFP-positive, HRP-negative cells that had no obvious processes, and they were located more closely to the body surface than cell bodies of the da neurons. Identities of those cells are unknown. Scale bar: 50 μm. es, external sensory; oe, oenocytes; sp, spiracle.