Skip to main content
Figure 8 | Neural Development

Figure 8

From: Complex and dynamic patterns of Wnt pathway gene expression in the developing chick forebrain

Figure 8

Effects of Shh signalling on Wnt4 and Wnt5A expression in the developing forebrain. Lateral views of hemisected brains; anterior points to the right. (A-C) Expression of Shh (red) and Wnt4 (blue) at HH17 (A), HH18 (B) and HH22 (C). Note the widening gap between Wnt4 expression and basal plate (B, asterisk). (D-F) Wnt4 expression in the control (co) half of the brain (D) and in the half that was electroporated (ep) at HH14 with Shh and green fluorescent protein (GFP)-expressing plasmids (E,F) after 24 hours of incubation. Note the downregulation of Wnt4 on the electroporated side (asterisk, E). (F) Overlay with anti-GFP staining. (G-I) Wnt4 expression in the control (co) half of the brain (G) and in the half that was electroporated (ep) at HH15 with PtcΔloop2 (H,I) after 48 hours of incubation. Note the ectopic expression of Wnt4 in (H) in an area of the thalamus that has downregulated Wnt4 on the control side (asterisk, G). (I) Overlay with anti-GFP staining. Scale bars in (A-D,G) represent 0.1 mm. (J-L) Wnt5A expression in the control (co) half of the brain (J) and in the half that was electroporated (ep) at HH14 with Shh and GFP-expressing plasmids (K,L) after 48 hours of incubation. Note the dorsal expansion of Wnt5A expression in the prethalamic area (arrowhead, K). (M-O) Wnt5A expression in the control (co) half of the brain (M) and in the half that was electroporated (ep) at HH15 with PtcΔloop2 (N,O) after 48 hours of incubation. Note the patches of Wnt5A downregulation in the ventral midbrain, ventroposterior diencephalon and posterior telencephalon (black arrowheads, N). Note the Wnt5A upregulation in a few cells in the thalamus (red arrowhead, N). Arrows mark the ZLI in (M,N). Scale bars in (J,M) represent 0.2 mm.

Back to article page