Figure 3

Expression extending into the ventral forebrain - Wnt2B , Wnt5A , Wnt5B , Wnt7A , Wnt7B , Wnt8B and Wnt9A. (A-X) Expression of Wnt2B (A-C), Wnt5A (D-F,V), Wnt5B (G-I,W), Wnt7A (J-L), Wnt7B (M-O), Wnt8B (P-R,X) and Wnt9A (S-U) at HH13 (A,D,J,M,P), HH14 (G,S), HH15 (E,N), HH16 (B,H,K,Q,T), HH18 (I), HH19 (C,F,L), HH20 (O,R,U) and HH24 (double stainings with Shh in red, V-X). Lateral views of whole mount (A,D,J,M,P,S) or hemisected brains (B,C,E-I,K,L,N,O,Q,R,T-X) are shown; anterior points to the right. Note Wnt2B expression in the basal plate (arrow) and in the dorsal pallium at HH19 (arrowhead, C). The arrowhead in (F) highlights Wnt5A expression flanking the zona limitans intrathalamica (ZLI) at HH19. Note the expression of Wnt5B along the diencephalic dorsoventral interface at HH16 (arrowhead, H) and, at HH18, in the prethalamus (asterisk) and weakly at the DMB (arrowhead, I). The arrow in (O) marks the ZLI. Note the ventral patch of Wnt8B expression posterior to the hypothalamus between HH13 and HH16 (arrowheads, P,Q). Note the thalamic expression of Wnt9A lining up posterior to the prospective ZLI at HH16 (arrowhead, T). The arrowheads in (V-X) mark the evaginating pineal gland. Note the expression of Wnt5B in the thalamus at HH24 in a fine line parallel to the alar plate-basal plate interface (arrow, W). Scale bars represent 0.2 mm. Abbreviations: bos, base of optic stalk; hy, hypothalamus.