Table 2 Primary antibody information
From: Ptf1a is expressed transiently in all types of amacrine cells in the embryonic zebrafish retina
Antibody | Dilution | Source | Immunogen | Specificity |
|---|---|---|---|---|
rb anti-calretinin | 1:2000 | Millipore, AB5054 | Recombinant rat calretinin | Immunoblot (manufacturer) |
rb anti-GABA | 1:500 | Sigma, A2052 | GABA-BSA | Dot-blot analysis (manufacturer) |
ms anti-GFP | 1:500 | Roche, 11814460001 | Partially purified recombinant Aequorea victoria GFP | Western blot and immunoprecipitation (manufacturer) |
rb anti-glutamine synthetase | 1:50 | Millipore, MAB302 clone GS-6 | Glutamine synthetase purified from sheep brain | Western blot of sheep and rat brain (manufacturer) |
rb anti-glycine | 1:200 | Millipore, AB139 | Glycine-glutaraldehyde-BSA | Free floating PAP technique on rat spinal cord (manufacturer) |
rb anti-Hermes | 1:400 | Associate Professor Malgorzata Kloc., University of Texas, USA | Recombinant His-tagged Hermes protein | Western blot to recognise my-tagged Hermes fusion protein [66] |
rb anti-PKCβ1 | 1:150 | Santa-Cruz Biotechnology, (C-16) sc-209 | Carboxyl terminus of PKC β1 of human origin | Western blot in NIH/3t3, 3611-RF, A-431, HeLA and Jurkat whole cell lysate (manufacturer) |
rb anti-prox1 | 1:200 | Millipore, MAB5652 clone 5G10 | Recombinant human Prox1 protein | Western blot in fetal mouse brain lysate (manufacturer) |
ms anti-parvalbumin | 1:1000 | Millipore, MAB1572 clone parv19 | Frog muscle parvalbumin directed against epitope at first Ca2+ binding site | Western blot (manufacturer) |
rb anti-sox2 | 1:200 | Millipore, MAB5603 | Synthetic peptide corresponding to human Sox2 | Western blot of whole cell or nuclear extracts (manufacturer) |
ms anti-5E11 | 1:50 | Associate Professor James M Fadool, Florida State University, USA | Whole zebrafish retina (antibody was subsequently purified) | Western blot of total retina protein [67] |
ms anti-Zpr1 | 1:500 | ZIRC, Monoclonal Antibody Facility, University of Oregon | Adult zebrafish protein | Clones selected on basis of staining pattern |
ms anti-Zpr3 | 1:400 | ZIRC, Monoclonal Antibody Facility, University of Oregon | Adult zebrafish protein | Clones selected on basis of staining pattern |