Figure 7

Morphology of different types of amacrine cells in Ptf1a:GFP DNA-injected embryos (120 hpf). Micrographs show single images or extended focus views to reveal most of the morphology of individual cells without interfering neurites from neighbouring labelled cells. The nuclear stain DAPI was used to reveal the retinal layers. Their somas are found in the inner half of the INL or in the GCL immediately adjacent to the IPL. Their neurites can be smooth or beaded (with varicosities) and differ in width, stratification depth, and stratification breadth. (A-F) Narrow-field (NF) amacrine cell type examples that are monostratified (A, B), displaced (somas in the GCL in (C, D)) and monostratified, or multistratified (E, F). (G) Medium-field (MF) amacrine cell with varicose processes stratifying throughout the OFF sublamina. (H, I) Examples of two wide-field (WF) amacrine cells stratifying broadly in the OFF sublamina (H) or narrowly in stratum 3 (I). dAC, displaced amacrine cell; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; mono, monostratified; S, stratum. Scale bar = 10 μm.