Figure 2From: Glypican-1 controls brain size through regulation of fibroblast growth factor signaling in early neurogenesisAdult brain weight from Gpc1 null mutant is significantly reduced. (A) Dorsal views of freshly dissected adult brains. Note the apparent reduction in size of Gpc1-/- brains. (B, C) Wet weights of fresh adult brains. Gpc1 genotypes are as indicated. There is a 15.5% decrease in brain weight in homozygous Gpc1 mutants (P < 0.0001); heterozygotes show an intermediate phenotype (7.8% decrease; * and **P < 0.0001). (B) N = 32 for +/+; 29 for +/-; 36 for -/-. Reduced brain weight is also observed in Gpc1LacZmutants. (C) N = 9 for +/+; 10 for LacZ/+; 7 for LacZ/LacZ. (D) Body weights of adult Gpc1 mutant and wild-type littermates, grouped by sex. No significant effect of genotype is observed. N = 23 for +/+; 30 for +/-; 37 for -/-. (E) Total DNA content of mutant and wild-type brains was measured as an indication of cell number. The data imply a 20% reduction in Gpc1-/- brains. (*P < 0.001; N = 5 for +/+, and 5 for -/-).Back to article page