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Figure 9 | Neural Development

Figure 9

From: Dynamic Pax6 expression during the neurogenic cell cycle influences proliferation and cell fate choices of retinal progenitors

Figure 9

Effects of altering Pax6 expression on cell death. (A-D) Immunofluorescent images of retinal explants transfected at E4.5 (HH stage 24) with U6 control (A, B) and U6-Pax6i (C, D) vectors after 2 days in vitro. (A, C) Merged images for green fluorescent protein (GFP), activated caspase 3 (Casp3, red), and DAPI. (B, D) Merged images showing GFP+ transfected cells and Casp3+ signals. Note that Pax6i-transfected retinas show higher levels of apoptosis than the U6 control. Arrows in (D) indicate cells co-labeled for GFP and Casp3. (E, F) Quantification of Casp3+ cells among GFP+-transfected cells in dissociated E5 (HH stage 27) retinal cell cultures after 2 days in vitro. Statistically significant data points between the controls and given samples are indicated with asterisks and specific P-values (N = 3, mean ± standard error). gcl, ganglion cell layer; vs, ventricular surface.

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