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Figure 3 | Neural Development

Figure 3

From: Dynamic Pax6 expression during the neurogenic cell cycle influences proliferation and cell fate choices of retinal progenitors

Figure 3

Pax6 expression during S, G2 and M phase of the cell cycle. Merged confocal immunofluorescent images of E6.5 (HH stage 30) retinas after a 20-minute bromodeoxyuridine (BrdU) pulse label in vitro. (A-F) Co-staining of BrdU and Pax6 at 0 (A, D), 30 (B, E), and 60 minutes (C, F) after the pulse label. The boxed regions in (A-C) are shown in (D-F), respectively, at twofold magnification. Note the appearance of BrdU+ cells at the ventricular surface by the 30-minute chase time. (G, G', H, H') Co-staining of phospho-histone 3 (PH3) and Pax6 at 30 (G, G') or 60 minutes (H, H') after the pulse label. The boxed regions in (G, H) are shown as separate panels in (G', H'), respectively, at twofold magnification. Note the appearance of BrdU+PH3+ double-labeled cells at the ventricular surface by the 60-minute chase time. Arrows indicate BrdU and PH3 co-labeled cells. Arrowheads point to BrdU+ cells. gcl, ganglion cell layer; vs, ventricular surface; vz, ventricular zone. Scale bar: 100 μm; the scale bar in (A) applies to (A-C, G, H).

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