Differentiation of individually cultured neuroectodermal progenitor cells. (A-H) Cultured neuroectodermal progenitor cells produce between 2 and more than 20 neural progeny cells. Neurons show small round cell bodies and develop long fibre projections that sometimes fasciculate. Glia exhibit flat cytoplasmic protrusions with irregular shape. Besides general markers for neurons or glia (B) (anti-Elav, green; anti-Repo, red), some clones express markers characteristic for particular subsets of neurons ((F) anti-serotonin; (H) anti-Eve), for subsets of glial cells ((C) M84/P101-lacZ; (D) Mz97-GFP, arrows) or for particular lineages ((E) eagle-lacZ). Some progeny cells undergo programmed cell death as revealed by anti-activated Caspase 3 (G, arrow) or TUNEL staining (H) (see yellow spot (arrow) in distant cell of clone double-stained against Eve). Markers applied in (B-H) are indicated. The sizes of postmitotic progeny cells correspond to 5 to 6 μm.