Figure 5

Dynamic microtubules remain high in dendrites at 7 DIV. Comparison of immunofluorescence intensity in the mid- and distal dendritic shaft of neurons at 7 DIV shows both Ac-tubulin and Tyr-tubulin decline as the dendrites taper. At the mid-shaft, Tyr-tubulin is about 65% of its original intensity at the origination of the dendrite from the soma, whereas Ac-tubulin has decreased to 78%. These values are significantly different from each other (distinguished by different letters, A versus B, P < 0.05). Both Ac and Tyr have decreased to about 48% of the original intensity in distal regions (excluding the last 10 μm of distal tip/growth cone region). Error bars represent standard error of the mean.