Figure 3

Tyr-tubulin is increased in distal dendrites at stages of development when dendrite outgrowth is robust. The relative amount of change between mid-shaft and distal regions of dendrites was determined for both Tyr-tubulin and Ac-tubulin by determining the ratio of fluorescence intensity between the two locations for each antibody. At 2 DIV, both Ac and Tyr show a similar distribution from mid- to distal shaft, but Tyr becomes significantly increased at 4 and 7 DIV. Values were determined by dividing the fluorescence intensity at the mid-shaft by that of the distal region. Tests of significance were done using a Student's t-test: 2 DIV, n = 122 dendrites; 4 DIV, n = 94 dendrites; 7 DIV, n = 59; *P < 0.05; ***P < 0.0001. Error bars indicate standard error of the mean.