Figure 6

Injection of botulinum toxin B into wild-type zebrafish mimics the nsf^{st 53}phenotype. Whole-mount in situ hybridization for wild-type (WT) and WT + botox at 5 dpf is shown (n = 4 each). The expression of neuroendocrine markers in botox-injected WT fish is highlighted (black arrowheads). Relative transcript levels are shown for the heads of WT and WT + botox at 5 dpf (n = 8 each). Fold induction is relative to one control sample and taken to be 1.0. All other samples, including additional control and experimental samples, are normalized to this one wild-type sample. Sample variation is shown by error bars (standard error of the mean). Statistical analyses were done by unpaired t-test. *P < 0.05; **P < 0.01. NS = not significant.