Figure 5

Accumulated neuropeptide protein and the buildup of endoplasmic reticulum-like membranes in nsf^{st 53}zebrafish. The levels of (A, B) hypocretin and (C, D) NPY protein in wild-type (WT) and nsf^{st 53}zebrafish are shown (n = 8 each). Protein levels are increased in the soma (B, D). DAPI stained nuclei are also shown (A-D). Electron microscopy images from (E) wild-type and (F) nsf^{st 53}zebrafish (n = 3 each). For EM analysis, neuroanatomical landmarks (obtained from [50]) and the presence of dense-core vesicles (Figure 4I) were used to locate the hypothalamus. The budding of secretory vesicles from the trans-Golgi network is highlighted (E, red arrowhead). A build up of ribosome-bound ER-like membranes in nsf^{st 53}zebrafish is noted (F, red arrowhead). PM = plasma membrane.