Skip to main content
Figure 4 | Neural Development

Figure 4

From: Neuroendocrine transcriptional programs adapt dynamically to the supply and demand for neuropeptides as revealed in NSF mutant zebrafish

Figure 4

nsfst 53hypothalamus undergoes normal development and does not appear to initiate apoptosis. Apoptosis in (A) wild-type (WT) and (B) nsfst 535 dpf zebrafish (12 μm horizontal sections) is shown. Cell death is not observed in the hypothalamus (B), but is observed in olfactory terminals (B, white arrowhead). Photoreceptor auto-fluorescence in wild-type zebrafish is lost in mutant (B, white arrow). DAPI staining of wild-type (A, C, E) and nsfst 53zebrafish (B, D, F) at 5 dpf is shown. Immunocytochemistry for (C, D) Hu family proteins and (E, F) SV2 are shown for wild-type and nsfst 535 dpf zebrafish. All images are representative of at least 15 animals assayed. (G-I) Electron microscopy images from nsfst 53hypothalamic sections (n = 3). Cell bodies (G) and vesicle-filled synapse (H) resemble wild-type neurons (data not shown). Anatomical landmarks and (I) the presence of dark-cored secretory vesicles (red arrowhead) were used to confirm the location of the hypothalamus. N = nucleus. Note that the medially located cell bodies (Figure 4C, D, G) are framed on either side by lateral projections (Figure 4E, F, H) in both cryosection and EM images.

Back to article page