Figure 3
gnrh3 transcripts persist and protein levels are normal in nsfst 53zebrafish. Whole-mount in situ hybridization for gnrh3 expression in the olfactory placode in 5 dpf wild-type (WT) and nsfst 53control or morpholino-injected larva is shown (top panels). Immunocytochemistry for GnRH peptide levels is shown (bottom panels). Images are representative of at least four animals per genotype per treatment. Anatomical landmarks include the olfactory epithelium (OE) and the eye.