Figure 7

Lhx9 and Lhx1 do not change dI cell fate. In situ hybridizations with dI-specific genes Foxd3 (dI2), and Lhx2 and Atoh1 (dI1) were preformed on sections of embryos electroporated with (A, B) Lhx1-IRES-GFP, (C, D) Lhx9-IRES-nGFP and (E-H) Lhx9-IRES-GFP. Adjacent sections were used for GFP detection (A, E, G) and for in situ hybridization (B, F, H). Alternatively, GFP detection by antibody staining and in situ hybridization were performed on the same slide (C, D). Ectopic expression of Lhx1 does not activate the expression of FoxD3 (A, B). Ectopic expression of Lhx9 down-regulates the expression of Lhx2 (C, D) and Atoh1 (E, F) and does not affect Foxd3 expression (G, H). Arrows point to dI2 neurons (B, H) and dI1 neurons (D, F). Arrowheads point to V1 neurons (B, H). Scale bar: 150 μm.