Figure 5
From: Molecular components underlying nongenomic thyroid hormone signaling in embryonic zebrafish neurons
T4 did not affect Rohon-Beard cell sodium current amplitude at 24 hpf. (A) The 24 hpf zebrafish embryos were tested for the ability of Rohon-Beard cells (RBs) to respond rapidly to T4. INa peak amplitudes did not significantly change over 5 minutes of T4 application. In the representative recording, INa was elicited by a depolarizing voltage step to -10 mV from a holding potential of -80 mV. (B) At 24 hpf, 30 nM T4 application did not significantly alter RB INa (n = 6) compared to controls (n = 4). In contrast, when αVβ3 is expressed in the spinal cord at 48 hpf, T4 acutely increased RB INa (48 hpf data from [11]).