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Figure 2 | Neural Development

Figure 2

From: Molecular components underlying nongenomic thyroid hormone signaling in embryonic zebrafish neurons

Figure 2

Blockade of p38 or protein phosphatase 1 occluded T4-induced rapid increase in sodium current amplitude. (A) The traces show typical Rohon-Beard cell (RB) INa recordings elicited by a -10 mV depolarizing voltage step before (black) and after acute application of 30 nM T3 (green) or 30 nM T4 (red). The PD98059, SB203580, and okadaic acid (OA) treatment groups present RB INa recorded after 1 hour drug incubation. (B) Changes in INa peak amplitude over 5 minutes for either control, PD98059, SB203580, or OA showed that none of the treatments increased run-down of the current during the recording period. (C) Acute application of 30 nM T3 (n = 5) did not alter RB INa compared to control cells unexposed to acute hormone treatment. However, 30 nM T4 (n = 11) significantly increased peak INa amplitude over time in control RBs. PD98059 treatment (n = 6) did not significantly affect T4's increase in peak INa amplitude compared to controls. However, SB203580 (n = 5) or OA (n = 3) treatments prevented a rapid increase in INa amplitude (P < 0.05; ANOVA) in response to acute T4 application. The data presented were acquired in the presence of 125 mM extracellular Na+. Asterisks represent a p-value of < 0.05 and error bars represent standard errors.

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