Skip to main content
Figure 5 | Neural Development

Figure 5

From: Heterogeneity in the developmental potential of motor neuron progenitors revealed by clonal analysis of single cells in vitro

Figure 5

Clones derived from subtype-restricted ventral progenitors generate appropriate neuronal subtypes in vitro . (A-I) Immunohistochemical analysis of neuronal subtypes present in clones of ventral restricted progenitors with Chx10 (A), Hb9 (D), Isl1/2 (B, E, H) and Nkx2.2 (G) and Tuj1; (C, F, I) are merged panels with Tuj1 to label neurons. (J) Vertical dot plot of the number of neurons present in each neuronal subtype restricted clone: Chx10+ V2a interneurons (n = 11 clones), Hb9+ and Isl1/2+ MNs (n = 45 clones) and Nkx2.2+ V3 interneurons (n = 8 clones). (K-P) Representative images (bright phase and eGFP) of four- (K, L), six- (M, N) and eight- (O, P) day-old MN clones generated from a pMN-restricted progenitor derived from Hb9::eGFP transgenic mice. (Q) Vertical dot plot of neuronal number present in pMN-restricted clones from Hb9::eGFP mice after four (n = 25 clones), six (n = 32 clones) and eight (n = 45 clones) DIV. The percentage represents the fraction of clones that had more neurons than those counted two days before.

Back to article page