Figure 2

Sorted eGFP⁺ ventral progenitors from Nkx6.1::IRES::eGFP mice maintain regional identity markers immediately after plating. (A, B) Contour plots of dissociated cells from e9.5 trunks of wild-type (A) or Nkx6.1::IRES::eGFP^+/- mice (B). The logarithmic scale of eGFP fluorescence is on the x-axis and cell size on the y-axis (forward scatter). (C-J) Immunohistochemical analysis of sorted eGFP⁺ cells after attachment (approximately 2 hours after plating) with antisera for eGFP (D) and transcription factors expressed in different ventral progenitor domains, such as Nkx6.1 (C, E, G, I), Olig2 (H), Irx3 (F) and Nkx2.2 (J). Arrows point to progenitors from the p2 or p3 domains. (K) Proportion of sorted eGFP⁺Nkx6.1⁺ progenitors that express the pMN, p2, p3 or floor plate markers 2 hours after plating. (n = 20 wells from 2 experiments). (L) Proportions of three progenitor populations and floor plate within the Nkx6.1⁺ domain of e9.5 neural tube from 6 sections of brachial and thoracic segments (n = 3 animals). Note that the motor neuron progenitor population (pMN) is the most abundant. Bars represent mean ± s.e.m in all plots.