Figure 8

Postsynaptic density (PSD)-95 puncta are eliminated upon treatment with secreted Sema5B. (a-f) Representative time-lapse confocal images of a 9 to 10 days in vitro (DIV) hippocampal neuron transfected with green fluorescent protein (GFP) (a, d) and PSD-95-red fluorescent protein (RFP) (b, e) and treated with secreted Sema5B (50% bioactivity). (c, f) Merged images of (a), (b) and (d), (e), respectively. There was a significant reduction of the number of PSD-95-RFP puncta while little change was observed in neurite morphology (compare (c') with (f')). (g) Bath application of secreted Sema5B for 1 h resulted in an elimination of approximately 50% of PSD-95-RFP puncta. N = 4 cells per condition. Scale bar 25 μm.