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Figure 7 | Neural Development

Figure 7

From: Semaphorin 5B mediates synapse elimination in hippocampal neurons

Figure 7

There is a decrease in synapse number in cells treated with secreted Sema5B. (a-i) Confocal images of 9 to 10 days in vitro (DIV) hippocampal neurons treated with supernatant from hemaglutinin (HA)-Sema5BΔC-expressing human embryonic kidney (HEK)293 cells and immunolabeled with anti-postsynaptic density (PSD)-95 (a-c) and anti-synaptophysin (d-f) antibodies. Effective concentrations were determined by growth cone collapse assays on 1 DIV hippocampal neurons, and reflect concentrations that confer 25% or 50% of maximum growth cone collapse. (g-j) Synapse number was determined by the number of synaptophysin puncta that colocalized with PSD-95. N = 25 neurons from 4 separate cultures. Scale bar 20 μm.

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