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Figure 7 | Neural Development

Figure 7

From: Neuroligin1: a cell adhesion molecule that recruits PSD-95 and NMDA receptors by distinct mechanisms during synaptogenesis

Figure 7

Colocalization of Nlg1 and NMDA receptors prior to synapse formation. (A) Immunostaining of cortical neurons in culture at 4 and 10 d.i.v. with antibodies to Nlg1 (red), NR2A/B(green) and VGlut1 (blue). At 4 d.i.v., the majority of colocalized clusters of Nlg1 and NR2A/B were non-synaptic (arrowheads), whereas in 10 d.i.v. neurons the majority of colocalized NR2A/B and Nlg1 were found with the presynaptic marker VGlut1 (arrows). (B) The density of protein clusters as indicated in the legend are plotted over time in culture. Nlg/NR2 includes the clusters of NR2 (green circles) and Nlg1 (red squares) that colocalize with each other. The rest of the cluster densities in (B, C) are plotted similarly. Clusters of colocalized NR2A/B and Nlg1 were abundant at early time points in culture. The density of non-synaptic (ns) Nlg/NR2 positive clusters decreased with time. (C) The densities of presynaptic terminals and presynaptic terminals with Nlg1 and NR2 increased steadily over time in culture. Error bars are standard error. *P < 0.05, **P < 0.001; asterisks at the end of the curve refer to comparisons between 4 and 15 d.i.v.

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