Figure 9
Functional analysis of UNC-7 and UNC-9 in Xenopus oocytes. Left: superimposed traces of junctional currents evoked by 10 mV voltage steps from -100 to +100 mV in one of a pair of Xenopus oocytes injected with cRNAs for the UNC proteins indicated below. Right: graphs of conductance/transjunctional voltage (Gj/Vj) relations for initial conductance (G j0, open squares) and steady state conductance measured at the end of the 15 s pulse (Gj∞, closed squares) averaged from four to five cell pairs. Gj0 values are normalized to Gj0 at Vj = 0, while Gj∞ values are normalized to Gj0 at the same voltage (Gj∞/Gj0). Error bars represent standard deviation, n = 5. Results shown are for the following pairings, where relative polarity of Vj is defined with respect to the innexins on the right in each pair: (A) UNC-7S/UNC-7S; (B) UNC-7L/UNC-7L; (C) UNC-9/UNC-9; (D) UNC-7S/UNC-9; (E) UNC-7L/UNC-9; (F) UNC-7S/UNC-7L; (G) UNC-7S/UNC-7L+UNC-9, where equal quantities of UNC-7L and UNC-9 cRNA were co-injected into the right cell.