Figure 9

Analysis of Ccnd1^{D 2/D 2}and Ccnd1^hE/hEretinas at P0. (A-D) Expression pattern of PCNA) in Ccnd1^{D 2/D 2}retina (B), Ccnd1^hE/hEretina (D) and their respective wild-type (wt) controls (A, C) is shown. (E) Quantification of proportions of PCNA⁺ cells. (F-I) Expression pattern of NEFM in Ccnd1^{D 2/D 2}retina (G), Ccnd1^hE/hEretina (I) and their respective wild-type controls (F, H) is shown. (J) Quantification of NEFM⁺ cells. (K, L) TUNEL labeling in wild-type (K) and Ccnd1^hE/hEretina (L). (M, N) Activated CASP3 immunoreactivity in wild-type (M) and Ccnd1^hE/hEretina (N). Brackets in (B) show the 'apical gap' in the P0 Ccnd1^{D 2/D 2}retina. Abbreviations: NR, neural retina. Scale bar: 100 μm (N is representative for all panels).