Figure 8

Relationship between PTF1A⁺, BHLHB5⁺, and OTX2⁺ precursors. Retinal sections at (A-F) E12, (G-L) E14.5, and (M-R) P0 were double-labeled with combinations of antibodies against PTF1A, BHLHB5 and OTX2. Arrowheads in (C, F, I, L, O, R) show examples of cell co-expressing OTX2 and BHLHB5. Arrows in (B, C, E, F, H, I, K, L) point to the retinal pigmented epithelium (RPE). Abbreviations: DCL, differentiated cell layer; NBL, neuroblast layer; RPE, retinal pigmented epithelium; wt, wild type. Scale bar: 100 μm; (R) is representative for all panels.