Figure 6

Reduced densities of horizontal and amacrine cells in the Ccnd1^-/- retina. (A, C) Expression pattern of NEFM at P0 is shown. Arrows point to representative horizontal cells. Bright staining in the differentiated cell layer (DCL) is due to NEFM expression in retinal ganglion cells (RGCs). (B, D) Retinal whole mounts stained with NEFM antibody reveal differences in horizontal cell density across retina. Tissues were imaged from basal surface to reduce interference from NEFM immunoreactivity in RGCs. Insets show boxed regions. (E) Quantification of NEFM⁺ horizontal cells at P0. (F-K) Expression patterns of SOX2 (F, I) and ISL1 (G, J) at P0 (merged images in (H, K)) are shown. (L) Quantification of SOX2⁺, ISL1B⁺ amacrine cells at P0. Abbreviations: DCL, differentiated cell layer; NBL; neuroblast layer. Scale bars: 100 μm; (C) is representative for (A, C); (D) for (B, D).