Figure 2
From: Cyclin D1 fine-tunes the neurogenic output of embryonic retinal progenitor cells
Retinal progenitor cell (RPC) cell cycle is lengthened in the Ccnd1-/- retina. P0 wild-type and Ccnd1-/- retinas, cultured successively in iododeoxyuridine (IdU) for 2 hours and bromodeoxyuridine (BrdU) for 30 minutes, were triple-stained with mouse (A, D) anti-BrdU antibody (Ab) recognizing both IdU and BrdU, (B, E) rat anti-BrdU antibody recognizing only BrdU, and (C, F) with an antibody against PCNA marking RPCs. Arrows in (A-C) mark IdU+ only RPCs (IdU+, BrdU-; positive signal in (A, C) but not (B)) in wild-type retina that have moved up to the apical surface during the labeling period. Arrows in (D-F) mark IdU+ only RPCs (positive signal in (D, F) but not (E)) in the Ccnd1-/- retina during the same period. (G) Quantification of average RPC cell cycle time (Tc), S phase time (Ts) and G1 + G2 + M phase time (Tc - Ts) in wild-type (wt) and Ccnd1-/- retinas at E14.5 and P0. Scale bar: 50 μm; (F) is representative for (A-F).