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Figure 1 | Neural Development

Figure 1

From: Elevated P75NTR expression causes death of engrailed-deficient midbrain dopaminergic neurons by Erk1/2 suppression

Figure 1

Elevated P75NTRexpression is causal for cell death. (A) Quantitative RT-PCR of ventral midbrain tissue (VM) derived from EnDM(En1-/-;En2-/-) and EnHTE12 embryos, and of En1-expressing N2A cells inducible by doxycycline (Dox). P75NTRexpression is inversely correlated with En1 expression levels in tissues and cell lines. (B, C) Western blot analysis of the ventral midbrain tissue shows the same relationship between P75NTR protein levels and En1 expression. Each active En1 allele decreases the P75NTR expression level (n = 3, p = 0.002). (D) Ventral midbrain cultures derived from EnDMand En2-/- embryos. Silencing of P75NTRby double-stranded RNA oligos and application of P75NTR-inhibiting antibody (Rex) increases the survival rate of EnDMmesDA neurons compared to untreated control (Ctl) or after treatment with scrambled RNA oligos (n ≥ 6, p < 0.01). Error bars indicate standard error.

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