Figure 3

Expression of NM23-X4 and other Xenopus family members in embryogenesis. (A) Semi-quantitative RT-PCR analysis of NM23-X4 and NM23-X1 at stages 6, 9, 11, 15 and 18 of embryogenesis. Ornithine decarboxylase (ODC) was used as an internal control. (B) Whole-mount in situ hybridization of NM23-X4 with a probe containing the cDNA cloned in pBluescript as shown in the schematic. Expression is not detected until after gastrulation at neurula stages and with persistence in the head region. Shown are: (a) stage 3, lateral view, animal up; (b) stage 3, animal view (c) stage 18, dorsal view, anterior up; (d) stage 18, anterior view, dorsal up; (e) stage 29, dorsal up, anterior right; (f) stage 32, with enlarged view marked in dashed line and shown in (g); (h) stage 35, with enlarged view marked in dashed line and shown in (i); (j) stage 41. ot, otic vesicle; ph, pharyngeal arches; e, eye. (C) Whole-mount in situ hybridization of NM23-X1, -X2 and -X3 at representative tadpole stage 33: (k) NM23-X1 and (l) NM23-X2 are ubiquitously expressed, while (m) NM23-X3 is localized in the head region. Sense control is shown in (n).