Figure 5
From: Developmental patterning of glutamatergic synapses onto retinal ganglion cells
Spatial maps of dendritic and postsynaptic densities of RGCs across development. (a) Dendritic territories are determined by convolving a 10 μm diameter disk centered at every pixel of the image, with the dendritic skeleton (see Materials and methods). Within the dendritic territory of each cell, dendritic length (μm) per 100 μm2 (D/A), puncta per 100 μm2 (P/A), and puncta per micrometer of dendrite (P/D) were obtained. (b) Maps of local values of D/A, P/D, and P/A of OFF monostratified RGCs at different ages. Local densities were determined by counting puncta number and measuring dendritic (Dend) length within a sliding window with a diameter of 20 μm centered at each pixel of the image (see Materials and methods).