Figure 3
From: Developmental patterning of glutamatergic synapses onto retinal ganglion cells
PSD95-YFP clustering appears to reflect normal distribution of postsynaptic densities. (a) Retinal cross-section showing a P35 RGC transfected with td-Tomato and PSD95-YFP. The section was immunostained for CtBP2 (cyan). (b-e) Higher magnification of a dendritic branch from the cell in (a) showing colocalization of PSD95-YFP puncta (b, d; green) with CtBP2 puncta (b, e; blue). (f) Example of whole-cell recordings showing sEPSCs in untransfected (control) and transfected RGCs at P12. (g) Median amplitude and (h) mean frequency of each control and transfected RGC (open circles). The mean of each group is shown by a black circle. N = 8 cells for each group