Figure 8
From: Axonin-1/TAG-1 is required for pathfinding of granule cell axons in the developing cerebellum
Downregulation of AX-1 does not interfere with granule cell development or migration. Perturbation of AX-1 expression did not interfere with granule cell migration from the EGL through the ML. (a-d) No change in the distribution of Pax6-positive granule cells was detectable between untreated control embryos at HH35 (a) or HH38 (c) and embryos injected and electroporated with dsRNA derived from AX-1 at HH35 (b) or HH38 (d). (e,f) As expected, downregulation of AX-1 by ex ovo RNAi at HH34 did not interfere with proliferation of granule cell precursors, as AX-1 is expressed only after granule cells become postmitotic. Sagittal sections labeled with BrdU and counterstained with DAPI taken from control embryos (e) and embryos lacking AX-1 (f) at HH38 did not differ. Inserts in (b,d,f) show adjacent sections stained for EGFP to demonstrate that sections were taken from the electroporated area of the cerebellum. Bar: 50 μm.