Figure 7
From: Axonin-1/TAG-1 is required for pathfinding of granule cell axons in the developing cerebellum
Guidance but not growth of granule cell axons is affected in the absence of AX-1 in vivo . (a) Detailed analyses of coronal slices taken from control-injected embryos at HH35 revealed correctly formed parallel fibers expressing EGFP (arrows). (b,c) In contrast, EGFP-expressing granule cells in embryos injected and electroporated with AX-1 dsRNA and the EGFP plasmid did not extend their axons parallel to, but rather towards, the pial surface (open arrows). (c) Overlay with a neurofilament staining outlines non-electroporated parallel fibers in the developing ML. Bar: 25 μm.