Figure 7

Unequal segregation of Prospero/Miranda during symmetric division of IPs. Confocal images of representative CD8::GFP labeled clones (green) in (a, c) canonical non-DM or (b, d) DM lineages. Shown are mitotic figures of small NB-associated cells at anaphase/telophase, visualized by anti- PH3 staining of DNA (blue). Separate channels are also shown in insets for better contrast. The outline of the plasma membrane stained by CD8::GFP shows that both the GMC (a, c, arrowheads) and the IP (b, d, arrows) divide symmetrically and give rise to daughter cells of similar sizes. The dividing IP is identified by NB-like expression of Cyclin E during mitosis (b) while GMC division lacks Cyclin E expression at this phase of the cell cycle (a). In the mitotic GMC, Miranda distributes equally to both daughter cells (c, inset) while Prospero is nuclear (see Figure 3b). In IP division, Prospero and Miranda co-segregate to only one of the two daughter cells (d, insets). Scale bars: 10 μm.