Figure 4

Live imaging of multiple and repeated division of DM NB daughter cells in MARCM-labeled clones. Frames from time-lapse recordings of a DM clone labeled with CD8::GFP and tau::GFP in larval brain cultured over 13 hours. The large NB, not visible in these frames, divided twice during this time period (Additional data file 1). The time is indicated in minutes relative to the start of the recording. (a) Multiple divisions of small NB-associated cells may be ongoing simultaneously in the clone and each gives rise to two daughter cells of equal size (single and double arrowheads at following intervals). (b) A single NB daughter cell may undergo several rounds of division. Shown are two consecutive divisions of a cell outlined with dots. Following a first symmetric division (575'–675'), the lower daughter cell underwent a second division (710'–755') while its sibling did not divide further during the recording.