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Figure 5 | Neural Development

Figure 5

From: Forcing neural progenitor cells to cycle is insufficient to alter cell-fate decision and timing of neuronal differentiation in the spinal cord

Figure 5

Cell cycle exit is not required for initiation or progression of neuronal differentiation. (a-d) Immunodetection of Ngn2 (red) after 48 h of CyclinD1 forced expression. The transgenic cells located in the motor neuron differentiating field do not retain Ngn2 expression. (a-d) maximum projections; (c,d) high magnifications of the zone in the dotted lines in (a) and (b), respectively. The arrow marks a transgenic cell expressing Ngn2. (e-g) Single optical sections showing the co-expression of HuC/D (green) and P-H3 (red) in the ventral horn of a spinal cord 48 h after electroporation with a CyclinD2 expression vector. (h-k) Co-immunodetection of BEN/SC1/DM-GRASP (green) and P-H3 (red) 48 h after electroporation with CyclinD1. (h-k) Single optical sections. (i-k) High magnifications of the cell marked with an arrow in (h); the GFP channel is off. (j,k) Orthogonal sections along the mitotic cell.

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Neural Development

ISSN: 1749-8104