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Figure 2 | Neural Development

Figure 2

From: Forcing neural progenitor cells to cycle is insufficient to alter cell-fate decision and timing of neuronal differentiation in the spinal cord

Figure 2

Transgenic cells overexpressing CyclinD migrate into the differentiating field while remaining in the cell cycle. (a,b) Forty-eight hours following forced expression of a tagged-CyclinD2 version, cells expressing GFP (green) and the transgenic protein detected with an anti-Tag-V5 antibody (blue) are observed in the ventricular zone and differentiating field. (c,d) Detection of the cells in S phase visualized following a 30 minute pulse of BrdU (red). (e-i) Detection of the phospho-histone H3 (P-H3) on a cross-section of the spinal cord 48 h after overexpression of CyclinD2 (e,f, red) or D1 (g-i, blue). (h,i) Magnifications of the cell in anaphase shown in (g) (arrowhead). (a-f) Maximum projections of eight optical sections acquired at 5 μm Z steps; (g-i) single optical sections.

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