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Figure 3 | Neural Development

Figure 3

From: Neural tube derived Wnt signals cooperate with FGF signaling in the formation and differentiation of the trigeminal placodes

Figure 3

Wnt signals act in a temporal manner to regulate Pax3 expression. Explants isolated at HH10 and HH13 were cultured alone (A, C, E) or with SFRP2 (B, D, F) overnight and stained for Pax3 (A-D) and Wnt1 (E-F). Explants cultured with SFRP2 lost the expression of Pax3 (B), compared to controls (A). Cranial ectoderm (ce) isolated at HH13 (C, D) and cultured in the presence of SFRP2 (D) did not lose Islet1 expression compared to controls (C). Midbrain to rhombomere 2 (mb-r2) explants express Wnt1 when cultured alone (E) but lose endogenous Wnt1 transcripts when cultured in the presence of SFRP2 (F) (n = 4/4)

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Neural Development

ISSN: 1749-8104