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Figure 8 | Neural Development

Figure 8

From: Drosophila olfactory local interneurons and projection neurons derive from a common neuroblast lineage specified by the empty spiracles gene

Figure 8

ems loss of function leads to a loss of neurons derived from the lateral neuroblast (lNb). (A, C) Wild-type MARCM clones of GAL4-LN1, UAS-mCD8::GFP and GAL4-LN2, UAS-mCD8::GFP generated between 0–4 h after larval hatching. White arrows indicate Nb clones. (B, D) em s mutant clonal brains. Note that no cells are visible although the shrunk lobe (encircled by yellow dots) indicates that clones have formed and have been ablated due to loss of ems (yellow arrowheads). Scale bars, 20 μm. Genotype in (A): y, w, hsFLP/+ or Y; GAL4-LN1/UAS-mCD8::GFP; FRT82B tub-GAL80/FRT82B. Genotype in (B): y, w, hsFLP/+ or Y; GAL4-LN1/UAS-mCD8::GFP; FRT82B tub-GAL80/FRT82B, ems9Q 64. Genotype in (C): y, w, hsFLP/GAL4-LN2; UAS-mCD8::GFP/+; FRT82B tub-GAL80/FRT82B. Genotype in (D): y, w, hsFLP/GAL4-LN2; UAS-mCD8::GFP/+; FRT82B tub-GAL80/FRT82B, ems9Q 64.

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