Figure 3

Projection neurons (PNs) arise together with local interneurons (LNs) from the lateral neuroblast (lNb). (A) The entire lNb clone is visualised by tub-LexA::GAD; LexAop-rCD2::GFP (A1). D, dorsal; L, lateral. (A2) GAL4-GH146 cells within the lateral cluster; (A3) GABA staining; (A4) merge. (B) Single section to show a few cells of the lateral cluster in (A). The cell marked with the yellow asterisk is an example of a GABA-ergic cell belonging to the lateral cluster, but not labelled by GAL4-GH146. Cells such as these are likely to be LNs. The pink asterisk represents a cell that is GAL4-GH146 positive and does not stain for GABA and is identified as a PN. Scale bars, 10 μm. Genotype: Tub-LexA::GAD/+ or Y; FRTG13, hsFLP, Tub-GAL80/FRTG13, UAS-mCD8, GAL4-GH146; LexAop-rCD2::GFP/+.